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Spooling is:
Spooling is:
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Solution
The correct option is D Collection of isolated DNA
In recombinant DNA technology, in order to extract DNA from the cell, the cell is treated with various enzymes to dissolve the cell wall (if present), cell membrane and digest the macromolecules (proteins) associated with the DNA.
The purified DNA can be seen as a collection of fine threads which can be scooped and swirled around a glass rod or wooden spooling stick. This process is called spooling. This helps to isolate the purified DNA threads from solutions.
Fig: Spooling
Amplification of DNA - It refers to the generation of multiple copies of a specific sequence of DNA. It is done using the technique of PCR.
Cutting of separated DNA bands from the agarose gel during gel electrophoresis is called elution.
Transfer of separated DNA fragments to synthetic membranes is called Southern blotting.
In recombinant DNA technology, in order to extract DNA from the cell, the cell is treated with various enzymes to dissolve the cell wall (if present), cell membrane and digest the macromolecules (proteins) associated with the DNA.
The purified DNA can be seen as a collection of fine threads which can be scooped and swirled around a glass rod or wooden spooling stick. This process is called spooling. This helps to isolate the purified DNA threads from solutions.
Fig: SpoolingAmplification of DNA - It refers to the generation of multiple copies of a specific sequence of DNA. It is done using the technique of PCR.
Cutting of separated DNA bands from the agarose gel during gel electrophoresis is called elution.
Transfer of separated DNA fragments to synthetic membranes is called Southern blotting.
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