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Question
Stanley Cohen and _____A____developed recombinant DNA technology in___B_______.
Stanley Cohen and _____A____developed recombinant DNA technology in___B_______.
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Solution
The correct option is C A - Herbert Boyer, B - 1972
A DNA molecule containing fragments from more than one biological source that are normally not found associated with each other is referred to as recombinant DNA.
The construction of the first recombinant DNA was done by Stanley Cohen and Herbert Boyer in 1972. They extracted plasmid DNA (autonomously replicating circular extrachromosomal DNA) from E.coli and linked it to antibiotic resistance gene obtained from another plasmid of Salmonella typhimurium by making use of restriction enzymes and DNA ligase.
This recombinant DNA was then transferred to Escherichia coli, a host cell. The recombinant DNA could replicate using the new host’s DNA polymerase enzyme and made multiple copies of itself.
Kary Mullis invented the polymerase chain reaction (PCR). PCR is used to amplify small quantities of DNA.
A DNA molecule containing fragments from more than one biological source that are normally not found associated with each other is referred to as recombinant DNA.
The construction of the first recombinant DNA was done by Stanley Cohen and Herbert Boyer in 1972. They extracted plasmid DNA (autonomously replicating circular extrachromosomal DNA) from E.coli and linked it to antibiotic resistance gene obtained from another plasmid of Salmonella typhimurium by making use of restriction enzymes and DNA ligase.
This recombinant DNA was then transferred to Escherichia coli, a host cell. The recombinant DNA could replicate using the new host’s DNA polymerase enzyme and made multiple copies of itself.
Kary Mullis invented the polymerase chain reaction (PCR). PCR is used to amplify small quantities of DNA.
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