Question

State the main steps through which a bacterial plasmid gets converted into a transgenic plasmid containing human DNA.

Answer 1

Bacterial plasmid can be modified by any human gene or DNA.

• The gene of interest was selected which have to be inserted into the plasmid.
• Then the suitable bacterial plasmid was isolated.
• Plasmid was taken and treated with restriction enzymes to cut it at a specific point where the desired piece of DNA can be inserted.
• The DNA and the cut end of plasmid should complement each other.
• DNA and the plasmid was ligated with the enzyme ligase.
• The ligated plasmid then undergoes selection procedure.