1
Question
State whether the following statements are true or false.
1.A genetically modified Ti plasmid is non pathogenic but able to deliver desirable genes into plant cells.
2.The Ti plasmid depends on the host cell for replicating itself and creating multiple copies.
3.The foreign gene is inserted into the virulence/vir genes of Ti plasmid for transfer and integration into the plant cell.
State whether the following statements are true or false.
1.A genetically modified Ti plasmid is non pathogenic but able to deliver desirable genes into plant cells.
2.The Ti plasmid depends on the host cell for replicating itself and creating multiple copies.
3.The foreign gene is inserted into the virulence/vir genes of Ti plasmid for transfer and integration into the plant cell.
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Solution
The correct option is B 1 - T, 2 - F, 3 - F
Agrobacterium tumefaciens is a bacterial plant pathogen which carries the Ti Plasmid or Tumour inducing plasmid. The T-DNA segment of the Ti plasmid integrates itself into the plant cell’s genome to induce tumour formation. This property of the plasmid makes it an effective vector for transferring foreign genes into plant cells.
The T-DNA segment of the Ti Plasmid can be genetically modified to carry foreign DNA. A restriction enzyme can be used to cut the T- DNA segment at a specific site and the DNA fragment carrying the gene of interest can be inserted into this segment using ligase enzyme. Once modified, the T-DNA becomes incapable of inducing tumours. Thus a genetically modified Ti plasmid is non pathogenic.
The genes of Ti plasmid are responsible for the synthesis of proteins which can mediate the transfer and integration of the T-DNA into the genome of the host plant cells. These genes themselves do not get transferred or integrated into the plant cell genome. Hence the foreign DNA is not inserted into the virulence genes.
The Origin of replication (ORI) site present in a plasmid DNA makes it a self-replicating body. This is a specific sequence of nucleotides that is recognised by initiator proteins (helicases) during replication of DNA. Thus, ORI site is the point at which replication initiates in a DNA molecule.
The Ti plasmid has its own Origin of replication (ORI) site which makes it a self replicating body and hence does not depend on the host to multiply itself.
Agrobacterium tumefaciens is a bacterial plant pathogen which carries the Ti Plasmid or Tumour inducing plasmid. The T-DNA segment of the Ti plasmid integrates itself into the plant cell’s genome to induce tumour formation. This property of the plasmid makes it an effective vector for transferring foreign genes into plant cells.
The T-DNA segment of the Ti Plasmid can be genetically modified to carry foreign DNA. A restriction enzyme can be used to cut the T- DNA segment at a specific site and the DNA fragment carrying the gene of interest can be inserted into this segment using ligase enzyme. Once modified, the T-DNA becomes incapable of inducing tumours. Thus a genetically modified Ti plasmid is non pathogenic.
The genes of Ti plasmid are responsible for the synthesis of proteins which can mediate the transfer and integration of the T-DNA into the genome of the host plant cells. These genes themselves do not get transferred or integrated into the plant cell genome. Hence the foreign DNA is not inserted into the virulence genes.
The Origin of replication (ORI) site present in a plasmid DNA makes it a self-replicating body. This is a specific sequence of nucleotides that is recognised by initiator proteins (helicases) during replication of DNA. Thus, ORI site is the point at which replication initiates in a DNA molecule.
The Ti plasmid has its own Origin of replication (ORI) site which makes it a self replicating body and hence does not depend on the host to multiply itself.
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