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Question

Streptomycin resistance gene is transferred from Nicotiana tabacum to Nicotiana silvestrise by which of the following methods?

A
Somatic embryogenesis
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B
Embryo culture
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C
Cybridization
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D
Somatic hybridisation
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Solution

The correct option is C Cybridization
Fusion of the whole cell with the cytoplast or protoplast produces a hybrid cell called as cybrid and this process of fusion is called as cybridization. In cybridization, heterozygosity of extra-chromosomal material can be obtained, which has direct application in plant breeding. So, streptomycin resistance gene is transferred from Nicotiana tabacum to Nicotiana silvestris by cybridization and not by somatic embryogenesis, embryo culture and somatic hybridization. Thus, the correct answer is option C.

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