Study the following figures and identify the enzymes involved in steps I and II.
A
EcoRI and DNA Ligase
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B
Hind II and DNA Ligase
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C
EcoRI and HindII
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D
Restriction endonuclease and exonuclease
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Solution
The correct option is C EcoRI and DNA Ligase At step I , there is a restriction endonuclease enzyme which works at the palindromic sequence AATTGC and at step 2, there is a DNA ligase which joins the separated fragments.