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Question

Study the following steps which are followed during the process of gene therapy while treating a patient of SCID
(i) Retrovirus infects lymphocytes extracted from bone marrow of the patient and cultured.
(ii) Engineered cells are injected into patient's bone marrow.
(iii) Normal allele is inserted into a retrovirus.
(iv) Retrovirus makes a DNA copy of its RNA. This DNA carrying the normal allele gets inserted into the chromosome of the host cell.
Arrange the above given steps in correct sequence and select the correct answer.

A
(iii), (i), (ii) (iv)
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B
(iii), (i), (iv), (ii)
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C
(iv), (ii), (iii), (i)
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D
(iv), (iii), (i), (ii)
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Solution

The correct option is B (iii), (i), (iv), (ii)
While treating a patient of SCID, the normal allele is inserted into the retrovirus, it infects the lymphocytes, DNA with normal gene gets inserted, engineered cells are inserted in the patients bone marrow.
So, the correct option is '(iii), (i), (iv), (ii)'

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