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Question
Study the given figure and name the three phases of PCR indicated by 1, 2 and 3.
Study the given figure and name the three phases of PCR indicated by 1, 2 and 3.
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Solution
The correct option is A 1-Denaturation, 2-Annealing, 3-Extension
In PCR, many copies of required DNA is made using two sets of primers and DNA polymerase. There are 3 steps involved: - Denaturation: Separation of the two strands of double stranded DNA of interest by application of heat at 95°C for a minute.
- Annealing: two primers hybridize to either ends of each strand of the single stranded DNA. This process is carried out at 55°C for a minute.
- Extension: Taq DNA polymerase synthesises DNA between the two primers on each strand of the single stranded DNA at 72°C for 2 mins.
In PCR, many copies of required DNA is made using two sets of primers and DNA polymerase. There are 3 steps involved:
- Denaturation: Separation of the two strands of double stranded DNA of interest by application of heat at 95°C for a minute.
- Annealing: two primers hybridize to either ends of each strand of the single stranded DNA. This process is carried out at 55°C for a minute.
- Extension: Taq DNA polymerase synthesises DNA between the two primers on each strand of the single stranded DNA at 72°C for 2 mins.