Substrate level phosphorylation in TCA occurs when

Succinic acid changes to fumaric acid

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Fumaric acid changes to malic acid

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Succinyl CoA changes to succinic acid

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Oxalosuccinic acid changes to

α -

ketoglutaric acid

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Solution

The correct option is C Succinyl CoA changes to succinic acid
In the succinyl CoA synthetase reaction, the thioester bond between HS-CoA and the succinyl group is hydrolyzed and energy is generated, which is enough for synthesizing GTP from GDP+(P). GTP can transfer the phosphate group to ADP for ATP formation. Since ATP is generated in this reaction, without any involvement of the respiratory chain, this reaction is called substrate level phosphorylation.
So, the correct option is 'Succinyl CoA changes to succinic acid'.

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α

C O_{2}

\begin{matrix} I . C i t r i c a c i d & \to & α - k e t o g l u t a r i c a c i d I I . S u c c i n i c a c i d & \to & M a l i c a c i d I I I . M a l i c a c i d & \to & O x a l o a c e t i c a c i d I V . α - k e t o g l u t a r i c a c i d & \to & S u c c i n y l C o - A \end{matrix}

N A D^{+}

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