Question

Taq polymerase which is used for amplification of DNA is related with

• A. Hybridoma technique
• B. PCR technique
• C. Gene cloning
• D. rDNA technology

Answer 1

The correct option is B PCR technique

Fusion of an antibody-producing B cell with a myeloma cell (with the ability to grow in tissue culture and lack antibody chain synthesis) produce hybrid cell lines that have the capacity to produce monoclonal antibodies and to grow in tissue culture indefinitely, thereby providing a constant supply of specific monoclonal antibodies.

Gene cloning refers to the production of multiple copies of a piece of DNA after its insertion into a vector. The vector is taken up by a cell and cloning occurs as the vector replicates.

r-DNA technology is the introduction of foreign genetic material into the genome of an organism that was not present before using restriction enzymes, DNA ligase and vectors.

PCR - technique amplify the gene of interest in three steps namely denaturation of target DNA (thermal cycle to separate the DNA strands), annealing of primers to the ssDNA and polymerisation (extension of primer into complete DNA strand complementary to the template strand). Taq polymerase is DNA polymerase from a hot-springs bacterium, Thermus aquaticus, that can withstand the denaturing temperatures. Therefore, omits the need of adding DNA polymerase, after each cycle of replication, to the reaction mixture and allow the cycling to be continued without interruption in PCR machines.

So, the correct answer is option B.