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Question

Technique used to detect the DNA in a clone is

A
Polymerase chain reaction
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B
Gel electrophoresis
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C
Chromatography
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D
Autoradiography
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Solution

The correct option is D Autoradiography
A single stranded DNA or RNA joined with a radioactive molecule (probe) is allowed to hybridise to its complementary DNA in a clone of cells. It is followed by detection using autoradiography. The clone having the mutated gene will not appear on the photographic film, because the probe will not have the complementarity with the mutated gene.

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