Question

teo method of recombinant DNA technology

Answer 1

Dear student

Recombinant DNA technology involves :

1. Isolation of genetic material .
2. Restriction enzyme digestion
3. Amplification using PCR
4. Ligation of DNA molecules
5. Insertion of recombinant DNA into host
6. Obtaintaining foreign gene product
7. Downstream processing

Therefore any two methods of recombinant DNA technology are :-

1. Isolation of genetic material - It is the first step which involves the isolation of dessired gene of interest in its pure form i.e free from all macromolecules.
2. Restriction enzyme digestion- Restriction enzymes are also known as molecular scissors. They cut the DNA at specific locations. This whole reaction is called restriction enzyme digestion.

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