Question

Terminal transferase is used

• A. to add base at the 3' end of the DNA
• B. to add base at the 5' end of the DNA
• C. to carry out nick translation
• D. to transfer phosphate at the 3' end of the DNA

Answer 1

The correct option is A to add base at the 3' end of the DNA

Terminal transferase (TdT) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. Protruding, recessed or blunt-ended double or single-stranded DNA molecules serve as a substrate for TdT.

So the correct option is 'to add base at the 3' end of the DNA'.