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B
Side chains of the amino acids
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C
Amino groups of the amino acids
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D
Carboxyl group of the amino acids
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Solution
The correct option is B Side chains of the amino acids The enzyme active site is composed of the amino acid residues. Side chains of the amino acids are seen in the active site region of the enzyme. The difference in the identity, charge, and spatial orientation of the functional groups located there results in the difference in enzyme specificity.
So, the correct option is ' side chains of amino acids'.