The activity of α - amylase in endospore of barley germinating seed is induced by
The substrate for α-amylase is stored starch and the end product is free sugar, which is needed for the growth of emerging embryo. After imbibition α-amylase begins in the aleurone layer by the De novo pathway. If the embryo of the seed is removed prior to inbibition, however, no increase in α-amylase activity occurs, suggesting that a hormone or plant growth substance produced by the embryo is necessary for the synthesis of α-amylase. The plant growth hormone gibberellic acid (GA) is the messenger that triggers this response. GA is produced in the embryo and migrates to the aleurone layer, where the synthesis of α-amylase is stimulated.