Question

The antigen is treated once with antibody tagged with the fluorochrome in which of the following technique?

• A. Direct immunofluorescence technique
• B. Indirect immunofluorescence technique
• C. Northern blot technique
• D. None of the above

Answer 1

The correct option is A Direct immunofluorescence technique

A- Direct immunofluoroescence also known as primary immunofluorescence is a technique that uses a single antibody that is chemically linked to a fluorophore ( Fluorophore is a fluoroscent chemical compound that can re-emit light on excitation). The antibody recognizes the target molecule and binds itself to it. The fluorophore it carries can be detected by microscope.