Question

The areas of application of PCR include:

A. Production of monoclonal antibodies.
B. Insertion of recombinant DNA into an organism.
C. Diagnosis of a specific mutation.
D. Detection of plant pathogens.

• A. A B
• B. B, C
• C. C, D
• D. A, B, C, D

Answer 1

The correct option is C C, D

Answer is option C i.e. "C, D"

PCR is used for diagnosis of diseases in animals and plants, it is also used to identify mutations in DNA. Major applications of PCR are:

(1) Clinical Diagnosis

(2) DNA Sequencing

(3) Gene Manipulation and Expression Studies

(4) Forensic Medicine and (5) Gene Cloning.

While, Production of monoclonal antibodies is done by Hybridoma technology and insertion of recombinant DNA into organisms is done by transformation or transduction.