The best way to identify a cell as either prokaryotic or eukaryotic is to determine whether
Cell with definite nuclei are called Eukarytoic cells. Cell without definite are called prokaryotic cells. Nucleus with nuclear membrane, nucleolus and nucleoplasm is called true nucleus or definite nucleus. Prokaryotes are generally single celled organisms and eukayotes both single celled and multicellular organisms.
So, the correct option is ‘ whether the nucleus is true nucleus or not true nucleus’.