Question

The cell division is paused at metaphase for karotype analysis. This is done using

• A. Colchicine
• B. Benzene
• C. Salicylic acid
• D. None of the above

Answer 1

The correct option is A Colchicine

Karyotyping is the process by which cytogenetist take photographs of chromosomes in order to determine the chromosome complement of an individual, including the number of chromosomes and any abnormalies. Joe Hin Tjio was responsible for finding the approach of Karyotyping:

• Using cells in tissue culture
• Pretreating cells in a hypotonic solution, which swells them and spreads the chromosomes
• Arresting mitosis in metaphase by a solution of colchicine
• Squashing the preparation on the slide forcing the chromosomes into a single plane.
• Cutting up a photomicrograph and arranging the result into an indisputable karyogram.
  

So, the correct answer is 'Colchicine'.