Question

The chemicals used for staining and mounting the section of a leaf (to show stomata) respectively are

• A. Iodine and glycerine.
• B. Glycerine and safranin.
• C. Iodine and safranin
• D. Safranin and glycerine.

Answer 1

The correct option is D

Safranin and glycerine.

The correct option is D.

Explanation of the correct option:

• Staining is the process of using a stain to color cells and tissue in a sample, and mounting culminates in a permanent histological preparation on the table.
• The red dye safranin is often used to stain plant cell preparations and examine stomata under a microscope, and glycerine works well as a drying agent. It keeps the specimen from drying out.

Explanation of Incorrect options:

Option A:

• In biology, iodine is used as a starch indicator.

Option B:

• Glycerine is not used for staining purposes.

Option C:

• Iodine is used as a starch indicator, and safranin is not used for mounting.

Therefore, the chemicals used for staining and mounting the leaf section to observe stomata are safranin and glycerine.