Question

The chromosome separation during metaphase can studied by

• A. Phase contrast microscope
• B. Microscope
• C. X-ray technique
• D. Light microscope

Answer 1

The correct option is A Phase contrast microscope

Phase contrast microscopy is particularly important in biology. It reveals many cellular structures that are not visible with a simpler bright field microscope. These structures were made visible to earlier microscopists by staining, but this required additional preparation and killed the cells. The phase contrast microscope made it possible for biologists to study living cells and how they proliferate through cell division. After its invention in the early 1930s, phase contrast microscopy proved to be such an advancement in microscopy, that its inventor Frits Zernike was awarded the Nobel prize in 1953. So, the chromosome separation during metaphase can studied by phase contrast microscope and not by TEM, X ray technique and scanning electron microscope as, these techniques can not be used in case of living cells.