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Question

The confirmatory test most commonly used to diagnose AIDS is

A
ELISA
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B
Western blot
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C
ESR
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D
PCR
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E
None of the above
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Solution

The correct option is B Western blot
The confirmatory HIV test employs a western blot to detect anti-HIV antibody in a human serum sample. Proteins from unknown HIV-infected cells are separated and blotted on a membrane. Then, serum to be tested is applied, the free antibody has washed away and a secondary anti-human antibody linked to an enzyme signal is added. The stained bands then indicate the proteins to which the patient' serum contains antibody.
Thus, the correct answer is option B.

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