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Question
The correct order of steps in Polymerase Chain Reaction (PCR) is
The correct order of steps in Polymerase Chain Reaction (PCR) is
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Solution
The correct option is D Denaturation, Annealing, Extension
Polymerase chain reaction (PCR) is a method widely used in molecular biology to make several copies of a specific DNA segment.
PCR involves a process of heating and cooling called thermal cycling which is carried out by machine.There are three main stages: - Denaturing – The double-stranded template DNA is heated to separate it into two single strands.
- Annealing – The temperature is lowered to enable the DNA primers to attach to the template DNA.
- Extending – The temperature is raised and the new strand of DNA is made by the Taq polymerase enzyme.
Polymerase chain reaction (PCR) is a method widely used in molecular biology to make several copies of a specific DNA segment.
PCR involves a process of heating and cooling called thermal cycling which is carried out by machine.There are three main stages:
- Denaturing – The double-stranded template DNA is heated to separate it into two single strands.
- Annealing – The temperature is lowered to enable the DNA primers to attach to the template DNA.
- Extending – The temperature is raised and the new strand of DNA is made by the Taq polymerase enzyme.
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