Question

The different steps of recombinant DNA technology are given below randomly.(i) Isolation of the DNA fragments or genes to be cloned(ii) Introduction of the recombinant DNA into a suitable cell (usually E.coli) called host (transformation)(iii) Multiplication/expression of the introduced gene in the host(iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment(v) Insertion of the isolated gene in a suitable plasmid vectorWhich of the following represents the correct sequence of steps ?

• A. (i),(iii),(ii),.(iv), (v)
• B. (iii), (ii), (i), (v), (iv)
• C. (i), (v), (ii), (iv), (iii)
• D. (v), (i), (iii), (iv), (ii)

Answer 1

Answer: (C)

(i), (v), (ii), (iv), (iii)

• Steps of recombinant DNA technology;
  

1. Isolation of genetic material i.e; isolation of the DNA fragments or genes to be cloned
2. Restriction enzyme digestion
3. Amplification
4. Ligation
5. Insertion of recombinant DNA into host i.e; insertion of the isolated gene in a suitable plasmid vector.
6. Transformation i.e; Introduction of the recombinant DNA into a suitable cell (usually E.coli) called host (transformation).
7. Obtaining foreign gene product i.e; multiplication/expression of the introduced gene in the host.
8. Down streaming process i.e; Selection of the transformed host cells and identification of the clone containing the desired gene/DNA fragment.
   

• So, the correct option is 'Option C' .