Question

The distribution of two or more specific molecules within a cell can be studied by using

• A. Dark field microscope
• B. Flourescent microscope
• C. Phase contrast microscope
• D. Interference contrast microscope
• E. Bright field microscope

Answer 1

The correct option is B Flourescent microscope

Fluorescent microscopy can be used to track one or more fluorescent molecules in a cell. Some proteins like green fluorescent protein (GFP) are naturally fluorescent. Their fluorescent properties can be altered and/ or enhanced artificially. These proteins can then be tagged to other molecules, thus facilitating tracking of tagged molecules. Dark and bright field microscopic techniques are well suited to reveal surface features and out lines of the specimen. Various contrast microscopes increases contrast and reveals cellular features without need for elaborate staining procedures.