The correct option is D Temperature
Elongation of nascent polypeptide occurs by covalent attachment of successive amino acid residues which are carried to the ribosome and correctly positioned by its tRNA. The peptidyl transferase forms peptide bond between the carboxyl group of peptidyl tRNA present at P site and free amino group of peptidyl tRNA present at A site which means that elongation is enzymatic process. Enzymes are biological molecules that require neutral-pH, mild temperature, aqueous environment inside cells for their stability. Thus, among given options, elongation is affected by temperature only. So, correct answer is D.