The correct option is D Pachytene of prophase I
Recombinase is an enzyme which catalyses the exchange of genetic material during crossing over of the non-sister chromatids of the bivalent. It leads to recombination of the genetic material of the homologous chromosomes. This step occurs during the pachytene stage of prophase I of meiosis I.
In zygotene stage of prophase I of meiosis I, homologous chromosomes pair together to form the bivalent.
In diplotene stage of prophase I of meiosis I, homologous chromosomes of the bivalent start to separate from each other.
In anaphase I of meiosis I, homologous chromosomes are pulled apart to the opposite poles of the cell.