The enzyme that reduces the tension during the unwinding of DNA helix in front of the replication folk is
A
Topoisomerase
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B
Helicase
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C
Ligase
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D
Polymerase
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E
Endonuclease
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Solution
The correct option is B Topoisomerase Helicases move along the double-stranded DNA and separate the strands by breaking hydrogen bonds, using from ATP. DNA denaturation by helicases creates topological stress in the helical DNA structure which is relieved by the action of topoisomerases. These enzymes transiently break both DNA strands of one chromosome thereby, making the other chromosome pass through the break and relieve the stress.
Ligase enzyme joins two large molecules by forming a new chemical bond; for example, DNA ligase joins two DNA fragments together via phosphodiester bond between two nucleotides.
Polymerase enzymes synthesize polymers by adding monomer units through specific bonding pattern. For example, synthesis of DNA is carried out by DNA polymerase enzyme that adds the deoxyribonucleotides to a primer in 5' to 3' direction via phosphodiester bonds.
Endonucleases are the enzymes that can break a large polymer into fragments by breaking the bonds within polymer; for example, restriction endonucleases recognize specific sequences within the DNA and cut it into small fragments. The correct answer is A.