The correct option is C 25-40∘C
Enzymes works precisely at certain narrowly optimum conditions, such as appropriate temperature, pH, and ion concentration. Deviation from the optimal conditions adversely affects enzyme activity. Most enzymes have an optimal temperature, at which the rate of reaction is fastest. For human enzymes, the temperature optima are near the human body temperature (35-40oC). Enzymatic reactions occur slowly or not at all at low temperatures. As the temperature increases, molecular motion increases, resulting in more molecular collisions. The rates of most enzyme-controlled reactions, therefore, increase as the temperature increases, within limits. High temperatures rapidly denature most enzymes. The molecular conformation (3-D shape) of the protein becomes altered as the hydrogen bonds responsible for its secondary, tertiary, and quaternary structures are broken. Because this inactivation is usually not reversible, activity is not regained when the enzyme is cooled.