Question

The enzymes which are absolutely necessary for recombinant DNA technology are :

• A. Restriction endonucleases and topoisomerases
• B. Endonucleases and polymerases
• C. Restriction endonucleases and Ligases
• D. Peptidases and Ligases

Answer 1

The correct option is C Restriction endonucleases and Ligases

• Recombinant DNA technology is the method which involves cutting and joining of different DNA fragments.
• This rDNA is then inserted into the host and allowed to produce the desired product.
• The restriction enzymes are the enzymes which cleaves the DNA at specific recognition sites.
• This helps in the production of fragments and desired genes. The enzyme ligases are used to join or ligate the fragments.
• These are two important enzymes with respect to rDNA technology.

Thus, the correct answer is option C.