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Question

The extension step of PCR where DNA polymerase gets into action happens at 72C. This is a contrast to what we have learnt where enzymes are said to be functioning at optimum temperature range of 35 to 40C. Why is it so?

A
DNA polymerase is active at low pH and high temperature
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B
While 36 to 40C is the optimum temperature range, 72C is a special sweet spot for most enzymes to work
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C
DNA polymerase used in PCR is obtained from a bacteria that thrives in hot springs
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D
The temperature has no role to play in this process.
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Solution

The correct option is C DNA polymerase used in PCR is obtained from a bacteria that thrives in hot springs
DNA polymerase enzyme used in the PCR is obtained from bacteria that is found in thermal vents and hot springs. They thrive in such high temperatures and hence72C. is the optimum temperature for their enzymes to work at. In fact, the high temperatures used to denature DNA which usually ranges from 95100C also does not denature this enzyme.

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