Question

The figure below is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly identifies its certain component(s)?

• A. amp${}^R$, tet${}^R$ - antibiotic resistance genes
• B. ori - original restriction enzyme
• C. rop - reduced osmotic pressure
• D. Hind III, EcoRI - selectable markers

Answer 1

The correct option is A amp${}^R$, tet${}^R$ - antibiotic resistance genes

E. coli clonning vector pBR322 shows:
Restriction sites - Hind III, EcoRI, BamHI, etc.
ori - origin of replication
Antibiotic resistance genes - amp${}^R$,
tet${}^R$
rop - for the proteins involved in the replication of the plasmid.