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Question

The figure below is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly identifies its certain component(s)?


A
ampR, tetR - antibiotic resistance genes
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B
ori - original restriction enzyme
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C
rop - reduced osmotic pressure
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D
Hind III, EcoRI - selectable markers
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Solution

The correct option is A ampR, tetR - antibiotic resistance genes
E. coli clonning vector pBR322 shows:
Restriction sites - Hind III, EcoRI, BamHI, etc.
ori - origin of replication
Antibiotic resistance genes - ampR,
tetR
rop - for the proteins involved in the replication of the plasmid.

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