The figure below is the diagrammatic representation of the E.coli vector pBR322. Which one of the given options correctly identifies its certain components(s)?

Ori - original restriction enzyme

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rop - reduced osmotic pressure

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Hind III, Eco RI - selectable markers

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a m p^{R}, t e t^{R}

- antibiotic resistance genes

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Solution

The correct option is D $a m p^{R}, t e t^{R}$ - antibiotic resistance genes
$a m p^{R}$ and $t e t^{R}$ are the antibiotic resistant gene parts and act as selectable markers.
Ori represents the site of origin of replication,
rop represents the proteins that take part in the replication of plasmid.
Hind III, Eco RI are the recognition sites of restriction endonucleases.

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