The figure below shows three steps (A,B,C) of polymerase chain reaction (PCR). Select the option giving correct identification together with what it represents?
A
C-Denaturation at a temperature of about98∘Cseparating the two DNA strands
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B
B-Denaturation at a temperature of about50∘C
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C
C-Extension in the presence of heat stable DNA polymerase
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D
A-Annealing with two sets of primers
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Solution
The correct option is CC-Extension in the presence of heat stable DNA polymerase
From the above figure, it can be identified as C represents extension of DNA strands in the presence of heat stable DNA polymerase like Taq polymerase.