The correct option is A hardening
Hardening refers to the preparation of the plants for a natural growth environment. The plantlets that are grown from the callus cannot be directly transferred to the field. They have grown in a well controlled, protected, sterile environment and they are not ready to cope with the environmental conditions of the field. Therefore, it is essential that the in-vitro plantlets are well acclimatized before they are transferred to the field via ‘hardening’.