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Question
The first restriction endonuclease characterised was from the bacterium __i___ and was known as __ii__.
The first restriction endonuclease characterised was from the bacterium __i___ and was known as __ii__.
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Solution
The correct option is C i- Haemophilus influenzae, ii- Hind II
Restriction enzymes are a type of endonucleases that cleave the phosphodiester bonds present between consecutive nucleotides within or near to specific DNA sequences that they identify. These identifiable sequences are called recognition sequences that are generally palindromic in nature. They are also called “molecular scissors or biological scissors” because they cut the DNA into multiple fragments.
The first restriction endonuclease characterised was Hind II. It was isolated from the bacterium, Haemophilus influenzae. It recognises a specific sequence of 6 base pairs.
Besides Hind II, more than 900 restriction enzymes have been isolated from over 230 strains of bacteria each of which recognises different recognition sequences. For example, EcoR I and EcoR II have been isolated from the bacterium Escherichia coli.
Therefore, the first restriction endonuclease to be characterised was from the bacterium Haemophilus influenzae and was known as Hind II.
Restriction enzymes are a type of endonucleases that cleave the phosphodiester bonds present between consecutive nucleotides within or near to specific DNA sequences that they identify. These identifiable sequences are called recognition sequences that are generally palindromic in nature. They are also called “molecular scissors or biological scissors” because they cut the DNA into multiple fragments.
The first restriction endonuclease characterised was Hind II. It was isolated from the bacterium, Haemophilus influenzae. It recognises a specific sequence of 6 base pairs.
Besides Hind II, more than 900 restriction enzymes have been isolated from over 230 strains of bacteria each of which recognises different recognition sequences. For example, EcoR I and EcoR II have been isolated from the bacterium Escherichia coli.
Therefore, the first restriction endonuclease to be characterised was from the bacterium Haemophilus influenzae and was known as Hind II.
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