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Question

The following sequence contains the open reading frame of a polypeptide. How many amino acids will the polypeptide consist?

5' AGCATATGATCGTTTCTCTGCTTTGAACT-3'

A
4
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B
6
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C
10
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D
7
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Solution

The correct option is B 6
Protein synthesis always starts with a start codon. Start codon is ATG. Hence the protein production will start from the 6th base of the sequence in the given question. Now starting from the ATG, if we read in triplets, the codons are ATG, ATC, GTT, TCT, CTG, CTT, and TGA. Triplet TGA (UGA in RNA) is a STOP codon. Thus the protein synthesis will start form ATG and will end on CTT, which means the polypeptide will contain 6 amino acids.

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