Question

The gene ‘Rop’ present in Pbr322 cloning vector codes for?

Answer 1

Recombinant DNA :

1. Recombinant DNA is delivered to the host cell using cloning vectors for multiplication.
2. One of the earliest commonly used E. coli cloning vectors was the plasmid pBR322.
3. A plasmid is referred to as a cloning vector, or simply a vector when it is used to replicate a foreign DNA sequence.
4. Most frequently, plasmids or bacteriophages are used as cloning vectors.
5. The gene Rop, present in the pBR 322 cloning vector, codes for the proteins involved in DNA replication.
6. Any fragment of foreign DNA that is connected to the origin of replication (ori), which is a sequence in the vector from which the replication originates, can be replicated.