The correct option is C Removal of a ring of tissue external to xylem
Girdling experiment is used to identify the tissue through which the food is transported. In this experiment, a ring of (phloem) is removed from the wood by a technique girdling. Since the woody xylem part remains intact, water and nutrient reach the leaves.
So the correct answer is 'Removal of a ring of tissue external to xylem'.