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Question

The given diagram represents which of the following bioreactors?




A
Airlift bioreactor
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B
Sparged stirred-tank bioreactor
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C
Simple stirred-tank bioreactor
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D
Anaerobic bioreactor
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Solution

The correct option is C Simple stirred-tank bioreactor
Bioreactors are large vessels that are used for large scale culturing of cells (plant, animal or microbial) to obtain specific gene products for commercial use. It provides optimum growth conditions, such as, temperature, pH, nutrients, oxygen (aerobic bioreactors), etc., to optimize production.

Stirred tank bioreactors are one of the most commonly used bioreactors. These are engineered to ensure stirring of contents in the culture medium thoroughly.

These can further be of two types - simple stirred-tank bioreactors and sparged stirred-tank bioreactors.

The diagram given in the question represents a bioreactor with stirrer blades (stirred type). It lacks a sparger to sparge oxygen bubbles into the culture media, which is a characteristic of sparged stirred tank type bioreactors. Thus, it can be concluded to be a simple stirred tank type bioreactor.


Simple stirred-tank bioreactors are large, closed cylindrical vessels, usually made of steel. They have a curved base to facilitate the mixing of the reactants in the culture broth.

Its components include central shaft, impellers for stirring the contents, oxygen delivery system (aerobic bioreactor), foam control machine, temperature and pH control systems, cooling jacket, sampling ports for periodic withdrawal of samples for optimisation of conditions.

An airlift bioreactor is a tower reactor where the mixing of the culture broth is done by the gas flowing in via an airlift pump.

The biosynthesis of certain desired products requires the host cells to grow in the absence of oxygen, that is, in an anaerobic condition. In such cases, specially designed anaerobic bioreactors are used.

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