Question

The graph given shows the effect of substrate concentration on the rate of reaction of the enzyme phosphatase from green gram. What does the graph indicates?

• A. The rate of enzymes reaction is directly proportional to the substrate concentration.
• B. Presence of an enzyme inhibitor in the reaction mixture.
• C. Formation of an enzyme substrate complex.
• D. At higher substrate concentration, the pH increase.

Answer 1

The correct option is A The rate of enzymes reaction is directly proportional to the substrate concentration.

The curve shows the effect of substrate concentration on the rate of enzyme catalyzed reaction. It shows that increasing substrate concentration increases the rate of reaction until a point comes when all the active sites of enzyme molecules are saturated with substrates and increasing the substrate concentration has no effect on the rate of reaction. The presence of enzyme inhibitor would reduce the reaction rate. The formation of enzyme complex is shown in the graph of the free energy level of the reaction mixture to the progress of the reaction. The graph is showing the relation between the rate of reaction and substrate concentration, not the pH of the reaction mixture. Thus, the correct answer is option A.