The correct option is C It is the energy required to bind the free nucleons to form a nucleus.
Binding energy is energy required to break a given nucleus into its free nucleons.
In other words, It is the energy released when the free nucleons combine to form a nucleus.
Also, B.E=mass defect×c2
Where, mass defect is the difference between the sum of the masses of the nucleons and the mass of the nucleus.
So, Option A,B and D are correct statement about binding energy.
Hence, option (C) is incorrect statement.