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Recombinant DNA Technology

The insertion...

Question

The insertion of transferred DNA into the plasmid can be tested by checking for the antibody - resistance of the bacteria with the plasmid.

A

False

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B

True

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Solution

The correct option is A False

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Q. From the below list, which of the following is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
(i) Transformbacteria with recombinant DNA molecule.
(ii) Cut the plasmid DNA using restriction enzymes.
(iii) Extract plasmid DNA from bacterial cells.
(iv) Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
(v) Use ligase to seal plasmid DNA to nonplasmid DNA.

Q. Which of the following contain plasmids, which can be used to insert DNA fragments into cells, for transgenic animals production?

Q. A piece of foreign DNA was inserted into a plasmid with an antibiotic resistance gene and lactose Z gene. The plasmid DNA was cut with a restriction enzyme, which splits the lactose Z gene and opens the circle. The foreign DNA was next inserted into the open restriction site of the plasmid. When the recombinant plasmid was introduced into bacterial cells and grown in the presence of antibiotic, some of the colonies turned blue in the presence of gene. The blue colonies contained

Q. This is figure of plasmid pBR 322. The gene conferring resistance to ampicillin (ApR) can be interrupted by insertion of a DNA fragment into the Pstl site, and the gene conferring resistance to tetracycline (TcR) can be interrupted by insertion of a DNA fragment into the BamHI site. Replication is controlled by the CoIEl origin. Use of the TcR and ApR genes allows for easy screening for recombinants carrying inserts of foreign DNA. For locating Eco RI, TcR and ApR gene on it. Identify, what is represented by A, B and C.

Q. Assertion :The Ti plasmid of Agro bacterium tumefactions has been effectively used as a vector for gene transfer in plants. Reason: Ti plasmids carry sites for insertion of foreign gene intended to be transferred.

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