Question

The introduction of t-DNA into plants involves

• A. Allowing the plant roots to stand in water
• B. Infection of the plant by Agrobacterium tumefaciens
• C. Altering the pH of the soil then heat-shocking the plants
• D. Exposing the plants to cold for a brief period

Answer 1

The correct option is B Infection of the plant by Agrobacterium tumefaciens

• Agrobacterium tumefaciens a plant pathogenic bacterium that carries Ti plasmid.
• It can transfer a particular segment of the tumor-inducing (Ti) plasmid into the nucleus of infected cells.
• The transferred T-DNA is then integrated into the host genome and transcribed with it.
• This ability of Agrobacterium tumefaciens to transfer the T-DNA in the host genome is explored in genetic engineering to transfer the desired DNA segment, carrying the gene of the interest, into the genome of selected organisms.
  Thus, the correct answer is option B.