The main function of the multiple cortical granules released from the oocyte into the perivitelline space is -
Prevent binding of additional sperm
Soon after the first sperm penetrates the zona pellucida of the ovum, calcium ions diffuse inward through the oocyte membrane triggering multiple cortical granules to be released by exocytosis from the oocyte into the perivitelline space. These granules contain substances that permeate all portions of the zona pellucida and prevent binding of additional sperm.