wiz-icon
MyQuestionIcon
MyQuestionIcon
12
You visited us 12 times! Enjoying our articles? Unlock Full Access!
Question

The method developed by Matthew Meselson and Franklin Stahl to separate heavy DNA with, 15N from DNA with l4N, for providing evidence for semi-conservative replication of DNA is

A
ion exchange chromatography
No worries! We‘ve got your back. Try BYJU‘S free classes today!
B
density gradient centrifugation
Right on! Give the BNAT exam to get a 100% scholarship for BYJUS courses
C
isopycnic centrifugation
No worries! We‘ve got your back. Try BYJU‘S free classes today!
D
gel filtration
No worries! We‘ve got your back. Try BYJU‘S free classes today!
Open in App
Solution

The correct option is B density gradient centrifugation
E. coli were grown for several generations in a medium with 15N. When DNA is extracted from these cells and centrifuged on a salt density gradient, the DNA separates out at the point at which its density equals that of the salt solution. The DNA of the cells grown in 15N medium had a higher density than cells grown in normal 14N medium. After that, E. coli cells with only 15N in their DNA were transferred to a 14N medium and were allowed to divide; the progress of cell division was monitored by microscopic cell counts and by colony assay.

flag
Suggest Corrections
thumbs-up
0
Join BYJU'S Learning Program
similar_icon
Related Videos
thumbnail
lock
Downstream Processing
BIOLOGY
Watch in App
Join BYJU'S Learning Program
CrossIcon