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Question

The method developed by Matthew Meselson and Franklin Stahl to separate heavy DNA with, 15N from DNA with l4N, for providing evidence for semi-conservative replication of DNA is

A
ion exchange chromatography
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B
density gradient centrifugation
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C
isopycnic centrifugation
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D
gel filtration
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Solution

The correct option is B density gradient centrifugation
E. coli were grown for several generations in a medium with 15N. When DNA is extracted from these cells and centrifuged on a salt density gradient, the DNA separates out at the point at which its density equals that of the salt solution. The DNA of the cells grown in 15N medium had a higher density than cells grown in normal 14N medium. After that, E. coli cells with only 15N in their DNA were transferred to a 14N medium and were allowed to divide; the progress of cell division was monitored by microscopic cell counts and by colony assay.

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