Question

The mRNA from a person is isolated and then a single stranded radiolabelled cDNA is synthesized using mRNA as template (Reverse transcriptase) and the radiolabelled cDNA is then allowed to hybridize with a known strand of DNA.Which one of the following images describe the above experiment?

• A. PCR
• B. RT - PCR
• C. Gel electrophoresis
• D. DNA Microarray

Answer 1

The correct option is D DNA Microarray

The polymerase chain reaction (PCR) is a technique used to amplify a single copy or a few copies of a piece of DNA to millions of copies of that particular DNA sequence.
RT-PCR is a technique where a mRNA is taken and then reverse transcriptase acts on it and a cDNA is created which then further acts as a template to PCR
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge.
The mRNA from a person is isolated and then a single stranded radiolabelled cDNA is synthesized.This radiolabelled cDNA is then allowed to hybridize with single stranded DNA attached to a solid surface.This DNA on the solid surface of the chip is specific(specified by researcher) .This whole process is called DNA microarray.