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Question

The number of phosphodiester bonds and hydrogen bonds broken due to action of EcoRI at one recognition sequence?

A
4&16
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B
2&4
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C
2&8
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D
6&8
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Solution

The correct option is C 2&8
The recognition site for EcoRI is 5’-GAATTC-3’ and the action of the enzyme nicks 2 phosphodiester bonds between G and A in the backbone of the two strands of DNA. Along with the phosphodiester bonds, a total of eight hydrogen bonds are broken in between the two strands, i.e. the bonds between A and T connecting the two strands.

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