The number of phosphodiester bonds and hydrogen bonds broken due to action of EcoRI at one recognition sequence?

4 & 16

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2 & 4

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2 & 8

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6 & 8

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Solution

The correct option is C $2 & 8$
The recognition site for EcoRI is 5’-GAATTC-3’ and the action of the enzyme nicks 2 phosphodiester bonds between G and A in the backbone of the two strands of DNA. Along with the phosphodiester bonds, a total of eight hydrogen bonds are broken in between the two strands, i.e. the bonds between A and T connecting the two strands.

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