The pKa values for the three ionizable groups X, Y and Z of glutamic acid 4.3,9.7and2.2 respectively. The isoelectric point for the amino acid is:
A
7.00
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B
3.25
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C
4.95
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D
5.95
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Solution
The correct option is D5.95 Isoelectric point (pI) is the pH at which the overall net charge of the amino acid is zero. So, the average of pKa values relevant to the protonation / depronation of the form with no net charge gives pI.